Glutathione-Bound Gold Nanoclusters for Selective-Binding and Detection of Glutathione S-Transferase-Fusion Proteins
نویسندگان
چکیده
This journal is (c) The Royal Society of Chemistry 2009 Glutathione-Bound Gold Nanoclusters for Selective-Binding and Detection of Glutathione S-Transferase-Fusion Proteins from Cell Lysates Cheng-Tai Chen, Wei-Jen Chen, Chao-Zong Liu, Ling-Ya Chang, Yu-Chie Chen Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan Department of Pharmacology and Institute of Pharmacology & Toxicology, Tzu Chi University, Hualien 970, Taiwan
منابع مشابه
Glutathione-bound gold nanoclusters for selective-binding and detection of glutathione S-transferase-fusion proteins from cell lysates.
A straightforward method for the rapid detection of the presence of glutathione S-transferase (GST)-tagged proteins from sample solutions using glutathione (GSH)-bound gold nanoclusters (Au@GSH NCs) with luminescence properties as the detection probes by simple observation with the naked eye was proposed in this study.
متن کاملProduction and Evaluation of Polyclonal Rabbit Anti-Human p53 Antibody Using Bacterially Expressed Glutathione S-transferase-p53 fusion protein
p53 is a key tumor suppressor gene that is targeted for inactivation during human tumorigenesis. In this study, we produced and characterized polyclonal antihuman p53 antibody. The cDNA encoding the completehuman p53 protein was cloned into pGEX-4T-1 and expressed in Escherichia coli as a fusion protein with Schistosoma japonicum glutathione S-transferase (GST). The rabbits were immunized...
متن کاملGlutathione S-transferase fusion proteins as an affinity reagent for rapid isolation of specific sequence directly from genomic DNA.
We describe a DNA binding assay for isolation of specific sequence(s) recognized by protein of interest directly from genomic or cosmid DNA. In our assay, the protein is fused to the glutathione-S-transferase and bound to glutathione-Sepharose beads. Then the immobilized fusion protein can be used to search for DNA fragment(s) that interact specifically with the protein of interest. As an examp...
متن کاملPurification Systems Based on Bacterial Surface Proteins
Affinity purification is based on the selective and reversible interaction between two binding partners, of which one is bound to a chromatography matrix and the other may be either a native target protein or a recombinant protein fused with an affinity tag (Cuatrecasas et al. 1968). Recombinant DNA-technology allows straightforward construction of gene fusions to provide fusion proteins with t...
متن کاملGlutathione S- transferases and their function as a protein superfamily in plants
Glutathione s transferase (GST) is one of the largest protein and multigene families present in all plant species and other living organisms. For these proteins, which are highly inducible to stress and internal and external stimuli, several functions in plants have been identified, including implication in secondary metabolism, growth and development, detoxification of herbicides, coping with...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2009